高效液相色谱法测定白花蛇舌草中异高山黄芩素的含量

• 作 者: 曹广尚 杨培民 周鹏
• 机 构: 山东中医药大学附属医院,
• 出 处: 中国医院药学杂志
• 发表日期: 2014年08期
• 关键词: 黄芩素 高效液相色谱法 白花蛇舌草 活性成分 线性关系 药效筛选 药材标准 对照品溶液 广西中药志 对照品

目的:建立HPLC法测定白花蛇舌草中新活性成分异高山黄芩素含量的方法。方法:采用Kromasil C18(150 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸(45∶55),检测波长280 nm,流速1.0ml·min-1,柱温30℃。结果:异高山黄芩素在13.2~145.2μg·ml-1范围内线性关系良好(r=0.999 9)。异高山黄芩素的平均加样回收率为99.77%(RSD=0.69%)。结论:该方法简便,准确,可靠,可用于白花蛇舌草中异高山黄芩素的含量测定。

OBJECTIVE: To establish a HPLC method for the determination of isobaric baicalein in Hedyotis diffusa. Methods: Kromasil C18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-0.1% phosphoric acid (45:55). The detection wavelength was 280 nm and the flow rate was 1.0 ml · min-1. Results: Baicalein was linear in the range of 13.2 ~ 145.2μg · ml-1 (r = 0.999 9). The average recovery of iso-baicalein was 99.77% (RSD = 0.69%). Conclusion: The method is simple, accurate and reliable and can be used for the determination of baicalein in Hedyotis diffusa.