论文部分内容阅读
取雄性大鼠(180～200g),先在侧脑室埋植不锈钢套管,在23±1°室温单只饲养,5～7天后供实验用。侧脑室内注射(icv)蛉蟾肽(Bombesin,Ⅰ)0.10μg(以生理盐水溶解,注射3～4μl,随后注入0.5～1μl 生理盐水冲洗)后,1min 内动物出现后肢猛力抓搔面、头和颈部,作用持续至少45min。将大鼠单只放进有机玻璃观察盒(长22cm,宽18cm,高25cm)内,借助微型计算机每次同时定量观察4只大鼠,每20sec 计数5sec 内出现擦身、搔痒或口咬的次数,共观察30min。注射(Ⅰ)后,每鼠30min 内最多搔抓数达90次。对照鼠(皮下注射及icv 生理盐水)的搔抓最多次数%为8±2(n=20)。测得的绝对搔抓次数以ANOVA 统计并进行t 测验,与对照组比较p<0.05者为有效。通过减去对照组的绝
Male rats (180 ~ 200g) were taken. The stainless steel cannula was implanted in the lateral ventricle first and only at room temperature of 23 ± 1 ° for experimental use after 5-7 days. After intracerebroventricular injection (icv) of Bombesin (Ⅰ) 0.10μg (dissolved in physiological saline, injected 3 ~ 4μl, followed by 0.5 ~ 1μl saline flush), within 1min, Head and neck, the role of sustained at least 45min. The rats were placed in a plexiglass observation box (22 cm in length, 18 cm in width and 25 cm in height). Four rats were simultaneously quantitatively observed with a microcomputer, scrubbing, itching or biting occurred every 5 seconds for 20 seconds The number of total observed 30min. After injection (Ⅰ), the maximum number of scratches in each rat within 30min was 90 times. Control mice (subcutaneous injection and icv saline) were found to have a maximum number of scratches of 8 ± 2 (n = 20). The absolute number of scratches measured was statistically analyzed by ANOVA and t test was p <0.05 compared with the control group were valid. By subtracting the absolute control group