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目的观察慢性丙型病毒性肝炎患者抗病毒治疗前后外周血中NK细胞、NK细胞亚群百分率及活化因子CD100表达水平变化。方法流式细胞术检测慢性丙型肝炎病毒(HCV)感染者外周血中NK细胞、NK细胞亚群频率及膜型CD100表达水平;ELISA检测血清中可溶性CD100水平;采用Spearman相关检验分析CD100与谷丙转氨酶(ALT)、HCV-RNA之间的相关性。结果与健康对照组相比,慢性HCV感染患者CD56dimNK亚群百分率降低,而CD56neg亚群比率升高(P<0.05);抗病毒治疗后获得早期病毒学应答(EVR)者,CD56bright亚群百分率升高,CD56neg亚群比率显著降低(P<0.05);停药后获得持续性病毒学应答(SVR)者,总NK细胞及亚群频率恢复正常。与健康对照组相比,慢性HCV感染患者血清中sCD100水平显著降低(P<0.05);膜型CD100表达轻度降低,但无统计学差异;经抗病毒治疗获得EVR者,sCD100及膜型CD100水平均显著升高(P<0.05),获得SVR者又降至正常水平。Spearman相关性分析发现,NK细胞CD100表达与ALT水平呈正相关,与HCV-RNA滴度呈负相关(P<0.05)。结论 CD100表达降低与HCV持续感染有一定关系,并参与NK细胞对HCV清除。
Objective To observe the changes of the percentage of NK cells and NK cell subsets and the expression of CD100 in peripheral blood of patients with chronic viral hepatitis before and after antiviral therapy. Methods The levels of NK cells and NK cells in peripheral blood of chronic hepatitis C virus (HCV) infected patients were detected by flow cytometry. The level of soluble CD100 in serum was detected by ELISA. Spearman correlation analysis was used to analyze CD100 and CD Aminotransferase (ALT), HCV-RNA between the correlation. Results Compared with healthy controls, the percentage of CD56dimNK subgroups in patients with chronic HCV infection was decreased, while the proportion of CD56neg subgroups was significantly increased (P <0.05). The percentage of CD56bright subgroups in patients with early HCV infection after antiviral treatment was significantly higher High, the ratio of CD56neg subpopulation was significantly lower (P <0.05); after discontinuation of sustained virological response (SVR), the total NK cells and subpopulations returned to normal. Serum levels of sCD100 in patients with chronic HCV infection were significantly lower than those in healthy controls (P <0.05), while membrane-type CD100 expression was slightly decreased but not statistically significant; patients with EVR, sCD100 and membrane-type CD100 after antiviral therapy The levels were significantly increased (P <0.05), SVR were reduced to normal levels. Spearman correlation analysis showed that CD100 expression in NK cells was positively correlated with ALT level and negatively correlated with HCV-RNA titer (P <0.05). Conclusions Decreased CD100 expression has some relationship with persistent HCV infection and is involved in the clearance of HCV by NK cells.