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目的:观察便秘模型小鼠肠道传输功能改变及加味桔梗汤的干预效果,为开展便秘“从肺论治”相应效应机制研究、探讨“肺合大肠”相关生物学基础奠定基础。方法:40只小鼠随机分为正常组、模型组、模型给药组及正常给药组,采用自身粪便与复方地芬诺酯灌胃法建立小鼠复合便秘模型。模型给药组及正常给药组以加味桔梗汤灌胃,正常组、模型组灌胃蒸馏水,连续7天,观察小鼠的排便情况及小肠推进率。结果:与正常组比较,模型组小鼠首粒黑便时间延长,4h排便粒数、4h排便重量、小肠炭末推进率均明显减少(P<0.01或P<0.05)。与模型组比较,模型给药组小鼠首粒黑便时间缩短,4h排便粒数、4h排便重量、小肠炭末推进率增加(P<0.05)。正常组与正常给药组未见显著性差异。结论:采用自身粪便与复方地芬诺酯灌胃法制备的小鼠复合便秘模型,存在明显肠道传输功能障碍;加味桔梗汤宣肺降气,可以促进胃肠蠕动、改善便秘小鼠肠道的传输功能。
OBJECTIVE: To observe the changes of intestinal transit function in mice with constipation and the intervention effect of Modified Campanulaceae, and to lay the foundation for the research on the corresponding mechanism of constipation and “lung treatment” and to explore the related biological basis of “lung combined with large intestine” . Methods: Forty mice were randomly divided into normal group, model group, model group and normal group. The mice model of compound constipation was established by using their own feces and diphenoxylate gavage. The model group and the normal group were given Jiawei Campanulaceae gavage, normal group, model group, intragastric administration of distilled water for 7 consecutive days to observe the mice defecation and intestinal propulsion rate. Results: Compared with the normal group, the mice in the model group were significantly longer in the first tablets of black stool, 4 hours of defecation, 4 hours of defecation weight, and small intestine carbon advancement (P <0.01 or P <0.05). Compared with the model group, the mice in the model group had shorter first melemic time, 4h defecation particles, 4h defecation weight, and small intestine charcoal propulsion rate (P <0.05). There was no significant difference between normal group and normal group. Conclusion: The compound constipation model of mice with self-defecation and diphenoxylate gavage has obvious intestinal transport dysfunction; Modified Campanulaceae Decoction can promote the gastrointestinal motility and improve the intestine of constipation mice Transfer function.