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用微相吸附-光谱修正（MPASC）新技术研究核酸与多色蓝（PCB）探针分子间的相互作用,分析核酸分子内双静电膜的形成与 Langmuir吸附的关联性.通过pH 7.24的介质核酸-PCB反应的光谱研究,测定了结合产物的物理化学参数:结合比IPCB:2DNA-PCB、IPCB:3RNA-PCB,平衡常数KDNA-PCB=5.42 X 104,KRNA-PCB=2.82 x 104,摩尔吸收系数ε（DNA-PCB,625nm）=5.65x103（mol-1·L）·cm-1,ε（RNA-PCB,625nm）=3.85x103（mol-1·L）·cm-1结果表明,RNA分子仅形成约60％双螺旋结构链,核酸双螺旋每一周期的负静电沟最大聚集10个PCB分子.该吸附反应用于核酸样品测定,结果良好.
The interaction between nucleic acid and multi-color blue (PCB) probe molecules was studied by using MPASC technique, and the association between Langmuir adsorption and the formation of bi-static films in the nucleic acid molecule was analyzed. The results of spectrophotometric analysis of nucleic acid-PCB reaction showed that the binding physico-chemical parameters of the binding products were as follows: binding ratio IPCB: 2DNA-PCB, IPCB: 3RNA-PCB, equilibrium constant KDNA-PCB = 5.42 X 104, KRNA-PCB = 2.82 × 104, Absorption coefficient ε (DNA-PCB, 625nm) = 5.65x103 (mol-1 · L) · cm-1 and ε (RNA- PCB, 625nm) = 3.85x103 (mol- 1 · L) The RNA molecules formed only about 60% double helix structure chain, and the double gap of the nucleic acid double helix cycle aggregated 10 PCB molecules at maximum, which was used for the determination of nucleic acid samples with good results.