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为比较新生儿脐带血和成人外周血淋巴细胞对抗 CD3单克隆抗体、PMA(Phorbol 1 2 - myristate 1 3- acetate)和 IM(Ionomycin)刺激的增殖反应 ,了解新生儿出生时淋巴细胞功能障碍是否与信号传导缺陷有关。方法 :流式细胞仪检测脐带血和外周血 CD3+、CD4+、CD8+T淋巴细胞的比率 ;3H- Td R掺入法检测淋巴细胞的增殖反应。结果 显示应用抗 CD3单克隆抗体或 PMA协同 IM刺激 ,新生儿脐带血中的淋巴细胞增殖反应明显增强 ,但脐带血淋巴细胞的增殖反应仍低于成人。因此 ,PMA协同 IM刺激可改善新生儿脐带血淋巴细胞增殖反应的缺陷 ,这可能与细胞信号传导的上游缺陷有关 ,提示细胞信号传导的障碍可能为新生儿出生时淋巴细胞功能低下的原因之一。
To compare the neonatal cord blood and adult peripheral blood lymphocytes against CD3 monoclonal antibodies, PMA (Phorbol 1 2 - myristate 1 3-acetate) and IM (Ionomycin) stimulated proliferation response to understand the neonatal lymphocyte dysfunction at birth whether And signaling defects related. Methods: Flow cytometry was used to detect the ratio of CD3 +, CD4 + and CD8 + T lymphocytes in umbilical cord blood and peripheral blood. 3H-TdR incorporation assay was used to detect the proliferation of lymphocytes. The results showed that anti-CD3 monoclonal antibody or PMA in combination with IM stimulation, neonatal umbilical cord blood lymphocyte proliferation was significantly enhanced, but the umbilical cord blood lymphocyte proliferation reaction is still lower than adults. Therefore, PMA combined with IM stimulation can improve the neonatal umbilical cord blood lymphocyte proliferation response defects, which may be related to the upstream defects in cell signaling, suggesting that the obstacles of cell signaling may be one of the causes of neonatal lymphocyte dysfunction at birth .