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同时应用定量PCR法和斑点杂交法对155例HBsAg阳性的慢性乙型肝炎患者的血清HBVDNA进行检测.以探讨两者的相互关系.结果显示斑点杂交法检出66例阳性(47.6%),定量PCR法检出110例(71.0%).HBV-DNA于2.54拷贝出现斑点杂交法阳性,随着HBV—DNA拷贝数的增加,斑点杂交法阳性增强,表明两种方法的阳性呈正相关关系.定量PCR法比斑点杂交法灵敏且可定量,但试剂昂贵;而斑点杂交法简便易行,适合基层单位应用.故建议临床宜先用斑点杂交法检测HBV—DNA,再配合定量PCR法以提高检出率并进行HBV-DNA定量.
Serum HBVDNA was detected by quantitative PCR and dot blot hybridization in 155 cases of HBsAg-positive chronic hepatitis B. The results showed that 66 cases (47.6%) were positive by dot blot hybridization, 110 cases (71.0%) were detected by PCR, and HBV-DNA was positive by Dot blot hybridization at 2.54 copies, with the increase of HBV-DNA copy number, the dot blot hybridization method was positive, indicating positive correlation between the two methods. PCR method is more sensitive and quantifiable than spot hybridization, but reagents are expensive. However, dot blot hybridization is simple and convenient and suitable for primary unit application. Therefore, it is suggested that HBV-DNA should be detected by dot blot hybridization first and then quantitative PCR Rate and carry out HBV-DNA quantification.