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目的:探讨苦参碱(Matrine)对雄激素依赖性前列腺癌细胞株(LNCaP)凋亡及前列腺特异抗原(prostates pecific antigen,PSA)表达的影响。方法:分别用0.5 g/L、1.0 g/L、1.5 g/L、2.0 g/L浓度的苦参碱作用于LNCaP细胞12 h、24 h、36 h后MTT法检测细胞生长活性;24 h后流式细胞仪测定细胞凋亡的变化;24 h后Western印迹法检测细胞内Bcl-2和Bax的表达;12 h、24 h、36 h后化学发光法检测LNCaP细胞培养液中PSA的变化。结果:苦参碱能抑制LNCaP细胞的生长,呈剂量与时间依赖性,不同浓度苦参碱组之间与不同作用时间组之间的差异均有统计学意义(P<0.05)。苦参碱诱导LNCaP细胞凋亡,各浓度组凋亡细胞比例均显著高于对照组,差异有统计学意义(P<0.05);LNCaP细胞内Bcl-2含量呈浓度依赖性下降,Bax含量呈浓度依赖性升高(P<0.01);LNCaP细胞培养液中PSA的表达显著下降(P均<0.05)。结论:苦参碱能显著抑制LNCaP细胞的体外生长,诱导其凋亡,并抑制PSA的表达。
Objective: To investigate the effects of matrine on the apoptosis and the expression of prostate specific antigen (PSA) in androgen-dependent prostate cancer cell line (LNCaP). Methods: LNCaP cells were treated with 0.5, 1.0, 1.5 g / L and 2.0 g / L matrine for 12 h, 24 h and 36 h, respectively. MTT assay was used to detect the cell growth activity. The changes of apoptosis were detected by flow cytometry. The expression of Bcl-2 and Bax was detected by Western blotting 24 h later. The changes of PSA in culture medium of LNCaP were detected by chemiluminescence after 12 h, 24 h and 36 h . Results: Matrine could inhibit the growth of LNCaP cells in a dose and time-dependent manner. The differences between matrine groups at different concentrations and at different time groups were statistically significant (P <0.05). Matrine induced the apoptosis of LNCaP cells, and the percentage of apoptotic cells in each concentration group was significantly higher than that in the control group (P <0.05). The content of Bcl-2 in LNCaP cells was decreased in a concentration-dependent manner. The content of Bax (P <0.01). The expression of PSA in LNCaP cell culture medium was significantly decreased (all P <0.05). Conclusion: Matrine can significantly inhibit the growth of LNCaP cells in vitro, induce apoptosis and inhibit the expression of PSA.