Construction and characterization of double mutants in nitrogenase of Klebsiella pneumoniae

来源 :中国科学通报：英文版 | 被引量 : 0次 | 上传用户：kukuhenku

【摘要】

：

Two mutants in nitrogenase of Klebsiella pneumoniae are constructed by site-directed mutagenesis and gene replacement procedure, which express the nitrogenases

【作者】

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ZHAODehua LIJilun

【机构】

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DepartmentofMicrobiology

【出处】

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中国科学通报：英文版

【发表日期】

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2004年16期

【关键词】

：

固氮酶突变异种克氏杆菌肺炎基质简化 Klebsiella pneumoniae double mutant activity of nitro-gena

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Two mutants in nitrogenase of Klebsiella pneumoniae are constructed by site-directed mutagenesis and gene replacement procedure, which express the nitrogenases with Lysine and Glutamine substituting for α-Glutamine 190 and α-Histidine 194 respectively (Kp

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Construction and characterization of double mutants in nitrogenase of Klebsiella pneumoniae

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