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目的观察烟碱型乙酰胆碱受体α7在Fmr1基因敲除小鼠前额皮层及海马的表达,探讨其在Fmr1基因敲除小鼠学习记忆中的作用。方法将4周龄FVB小鼠分为WT组和KO组,用跳台实验分别检测KO及WT小鼠学习记忆的能力,利用Western blotting和免疫组化方法检测KO及WT小鼠前额皮层及海马α7 nAChR的表达。结果跳台实验结果显示,与WT空白组比较,KO空白组第一天及第二天的潜伏期均显著缩短,错误次数明显增多,差异均具有统计学意义(P<0.05);免疫组化结果显示,与WT空白组相比,KO空白组前额皮层和海马区α7 nAChR免疫阳性细胞数均显著减少(P<0.05);Western Blotting结果显示,KO空白组前额皮层及海马区α7 nAChR表达量均较WT空白组减少,海马区更为显著,差异均具有统计学意义(P<0.05)。结论 Fmr1基因敲除小鼠前额皮层、海马区的α7 nAChR表达量较野生型小鼠减少,提示α7 nAChR参与了Fmr1基因敲除小鼠学习记忆障碍的发病机制。
Objective To observe the expression of nicotinic acetylcholine receptor α7 in Fmr1 knockout mice prefrontal cortex and hippocampus and to explore its role in learning and memory of Fmr1 knockout mice. Methods FVB mice of 4 weeks old were divided into WT group and KO group. The capacity of learning and memory in KO and WT mice was detected by jumping platform test. The expression of α7 in KO and WT mice prefrontal cortex and hippocampus were detected by Western blotting and immunohistochemistry nAChR expression. Results Compared with WT group, the latency of first day and second day of KO blank group was significantly shortened and the number of errors significantly increased, the difference was statistically significant (P <0.05); immunohistochemistry results showed , The number of α7 nAChR immunoreactive cells in the prefrontal cortex and hippocampus of KO blank group was significantly decreased compared with WT blank group (P <0.05). Western Blotting results showed that the expression of α7 nAChR in prefrontal cortex and hippocampus of KO blank group was significantly lower than that of WT blank group WT group decreased, the hippocampal area was more significant, the differences were statistically significant (P <0.05). Conclusions The expression of α7 nAChR in prefrontal cortex and hippocampus in Fmr1 knockout mice is decreased compared with that in wild type mice, suggesting that α7 nAChR is involved in the pathogenesis of learning and memory deficits in Fmr1 knockout mice.