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人乳头瘤病毒 (ＨＰＶ)感染与宫颈癌的关系已获公认。仍有许多学者采用原位杂交 (ＩＳＨ) ,斑点杂交和ＰＣＲ技术对其进行深入研究〔1,2〕。我们采用原位ＰＣＲ和ＩＳＨ技术对宫颈癌及宫颈上皮内瘤变 (ＣＩＮ)组织中的ＨＰＶＤＮＡ进行对比检测 ,探讨ＩＳＰＣＲ技术检测石蜡切
The relationship between human papillomavirus (HPV) infection and cervical cancer has been recognized. There are still many scholars using in situ hybridization (ISH), dot blot hybridization and PCR technology to conduct their in-depth study [1,2]. We use in situ PCR and ISH technology for cervical cancer and cervical intraepithelial neoplasia (CIN) tissue HPVDNA comparison test to explore the ISPCR technique to detect paraffin