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目的:探讨HSPTX(7.5%氯化钠+己酮可可碱)复苏对失血性休克大鼠诱发的急性肺损伤的影响。方法:24只雄性SD大鼠随机分为3组:假失血性休克(Sham)组,乳酸钠林格液(Ringer′s lactate,RL)复苏组,HS+PTX复苏组,每组8只。实验结束时测定各组大鼠肺水含量、支气管肺泡灌洗液(bronchoalveolor lavage fluid,BALF)中中性粒细胞(polymorphonuclear neutrophil,PMN)百分比和总蛋白(total protein,TP)浓度;测定肺组织髓过氧化物酶(myeloperoxidase,MPO)活性,检测肺组织中丙二醛(malondialdehyde,MDA)以及超氧化物歧化酶(superoxide dismutase,SOD)含量;ELISA法测定血浆中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素1β(interleukin1β,IL-1β)含量。结果:与Sham组相比,各实验组休克复苏2 h后肺水含量、总蛋白含量、PMN百分比、血浆中TNF-α、IL-1β表达以及肺组织MDA,MPO含量明显增加(P<0.01),SOD含量下降(P<0.01);HSPTX组大鼠肺水含量、PMN百分比、总蛋白含量、血浆中TNF-α、IL-1β表达以及肺组织MDA,MPO含量较RL组明显下降(P<0.01),SOD含量显著增加(P<0.01)。结论:HSPTX复苏可减轻失血性休克诱发的急性肺损伤。
Objective: To investigate the effects of HSPTX (7.5% sodium chloride + pentoxifylline) on acute lung injury induced by hemorrhagic shock in rats. Methods: Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups: sham group, RL group and HS + PTX group. There were 8 rats in each group. Lung water content, the percentage of polymorphonuclear neutrophil (PMN) and total protein (TP) in bronchoalveolar lavage fluid (BALF) were measured at the end of the experiment. The lung tissue The activity of myeloperoxidase (MPO), the content of malondialdehyde (MDA) and superoxide dismutase (SOD) in lung tissue were measured. The levels of tumor necrosis factor alpha factor-α, TNF-α) and interleukin 1β (IL-1β). Results: Compared with Sham group, lung water content, total protein content, PMN percentage, TNF-α and IL-1β in plasma and MDA and MPO in lung tissue were significantly increased 2 h after shock resuscitation (P <0.01). The content of lung water, the percentage of PMN, the content of total protein, the level of TNF-α and IL-1β in plasma and the contents of MDA and MPO in lung tissue of HSPTX group were significantly lower than those in RL group <0.01), SOD content increased significantly (P <0.01). Conclusion: HSPTX can relieve the acute lung injury induced by hemorrhagic shock.