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为进一步明确矮秆基因对杂交后代的致矮能力,一方面利用分子标记对26份小麦材料中所含有的矮秆基因(Rht-B1b、Rht-D1b、Rht8)进行了检测,另一方面选取较矮的8份材料作为母本,与两个高秆地方品种(中国春、宜宾白麦子)进行杂交,测量F1代株高并计算致矮力;最后,结合两方面的数据来估计矮秆基因对株高的影响.结果表明,同一母本与不同父本,或同一父本与不同的母本,杂交后F1代株高存在较大差异.因此,各矮秆基因表达可能具有遗传背景效应.在与Rht8紧密连锁的分子标记Xgwm261位点扩增出了3个等位变异,分别为174 bp、192 bp和210 bp,其中210 bp片段所占比例最高,为57.7%.特别值得注意的是,检测到1份同时含Rht-B1b+Rht-D1b+Rht8的材料(川育925矮).由于其株高矮(57.92 cm),平均致矮力高(29.4%),具高抗条锈病,且其它综合农艺性状好,因此推荐作为小麦矮化、半矮化育种的主要亲本之一.
In order to further clarify the dwarfing ability of dwarfing genes on hybrid progenies, we detected the dwarf genes (Rht-B1b, Rht-D1b, Rht8) in 26 wheat cultivars by using molecular markers on the one hand, Shorter 8 materials were used as the female parent to cross with two tall stalks (Chinese Spring and Yibin white wheat), F1 plant height was measured and dwarf power was calculated. Finally, the data from two aspects were used to estimate the dwarf The results showed that there was a big difference in plant height between the F1 and F1 hybrids between the same female parent and different male parents, or between the same male parent and the female parent.Therefore, the expression of each dwarf gene may have a genetic background Effect. Three allelic variants were amplified at the Xgwm261 locus closely linked to Rht8, which were 174 bp, 192 bp and 210 bp, of which the 210 bp fragment accounted for the highest proportion of 57.7%. , One piece of material (Chuan 925 short) with Rht-B1b + Rht-D1b + Rht8 was also detected.It has the highest resistance to shortness of force (29.4%) because of its short height (57.92 cm) Rust, and other comprehensive agronomic traits, it is recommended as one of the main parents of wheat dwarfing and semi-dwarf breeding .