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利用40个EST-SSR标记,对100份甘蓝自交系组成的自然群体进行遗传结构分析,采用TASSEL3.0软件的GLM(general linear model)和MLM(mixed linear model)模型,对甘蓝中心柱长和中心柱长/球高比值性状进行关联分析。结果显示供试材料可分为3个亚群;EST-SSR位点间有较高的多态性和一定程度的连锁不平衡;以GLM模型分析,共检测出5个标记的7个位点与中心柱长性状相关联,2个标记的3个位点与中心柱长/球高性状相关联,其中2个位点同时与两个性状相关联;以MLM模型分析,共检测出3个标记的4个位点与中心柱长性状相关联,1个标记的2个位点与中心柱长/球高性状相关联,其中2个位点同时与两个性状相关联。GLM和MLM两种模型同时检测到了与中心柱长/球高相关联的1个标记的2个位点以及与中心柱长相关联的3个标记的4个位点。
40 EST-SSR markers were used to analyze the genetic structure of 100 natural populations of cabbage inbred lines. The GLM (general linear model) and MLM (mixed linear model) And the center column length / ball height ratio correlation analysis. The results showed that the test materials could be divided into three subgroups: EST-SSR loci had higher polymorphism and linkage disequilibrium to a certain degree; with GLM model analysis, a total of seven loci Associated with the long trait of central column, three markers of two markers were correlated with the long / bulb trait of central column, of which two loci were related to two traits at the same time. Three MLM models were detected The four loci of the marker were associated with the long trait of the central column, and two sites of the one marker were associated with the long column / bulb trait of the central column, of which two loci were associated with two traits at the same time. Both the GLM and MLM models simultaneously detected 2 sites of 1 marker associated with the center column length / ball height and 4 sites of the 3 markers associated with the center column length.