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目的:研究益气升阳固齿方水煎液对炎症微环境下人牙周膜成纤维细胞生长及表达IL-6作用的调节作用。方法:人牙周膜成纤维细胞分别与脂多糖(LPS)50μg/ml、LPS 50μg/ml+益气升阳固齿方水煎液20g/l、LPS 50μg/ml+益气升阳固齿方水煎液40g/l、LPS 50μg/ml+益气升阳固齿方水煎液80g/l共同孵育6、72小时,于6小时收集细胞上清液,应用ELISA法检测IL-6表达水平;72小时应用MTT法检测存活细胞数量,评价细胞生长增殖水平,评价细胞生长增殖情况。结果:LPS可显著抑制人牙周膜成纤维细胞生长,并刺激IL-6表达;而益气升阳固齿方水煎液在40g/l以及80g/l下可显著抑制LPS刺激的IL-6表达。结论:益气升阳固齿方治疗牙周炎的机制可能与调节宿主过度的免疫反应有关。
Objective: To study the regulation effect of Yiqi Shengyang Guzhu decoction on the growth of human periodontal ligament fibroblasts and the expression of IL-6 in inflammatory microenvironment. METHODS: Human periodontal ligament fibroblasts were incubated with lipopolysaccharide (LPS) 50μg / ml, LPS 50μg / ml + Yiyangshenggucuang Decoction 20g / l, LPS 50μg / ml + The decoction of 40g / l, LPS 50μg / ml + Qi Shengyanggefang decoction 80g / l incubation with 6,72 hours, the cell supernatants were collected 6 hours, ELISA was used to detect IL-6 expression levels; The number of surviving cells was measured by MTT method, the proliferation and proliferation of cells were evaluated and the proliferation and proliferation of cells were evaluated. RESULTS: LPS significantly inhibited the growth of human periodontal ligament fibroblasts and stimulated the expression of IL-6. However, the effect of LQS Decoction at 40g / l and 80g / l significantly inhibited the LPS-stimulated IL- 6 expression. Conclusion: The mechanism of Yiyang Shenggu Decoction in treating periodontitis may be related to the regulation of host immune response.