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目的探讨二烯丙基二硫(DADS)对白血病细胞株 HL-60的生长抑制作用和对 HL-60细胞血管内皮生长因子(VFGF)mRNA 的表达及 VEGF 蛋白分泌的影响。方法采用 MTT 法观察DADS 对体外培养的 HL-60细胞的生长抑制;应用 ELISA 法检测药物作用前后 HL-60细胞培养上清液中 VEGF 蛋白的含量;半定量 RT-PCR 法检测药物作用前后 HL-60细胞 VEGF mRNA 表达水平。结果DADS 对 HL-60细胞具有明显的生长抑制效应,0.625,1.250和2.500μg/ml DADS 作用 HL-60细胞24 h 的细胞生长抑制率分别为(8.19±3.34)%,(16.79±2.07)%,(21.30±2.72)%;作用48 h 的细胞生长抑制率分别为(11.93±3.93)%.(22.81±2.31)%,(30.74±2.03)%;作用72 h 的细胞生长抑制率分别为(16.68±2.37)%,(28.54±3.26)%,(36.59±2.37)%,其对 HL-60细胞的生长抑制率与药物浓度有明显依赖关系(r>0.9,P<0.01),各实验组随作用时间增加抑制率明显增加(r>0.7,P<0.01);0.625,1.250,2.500 μg/ml DADS 作用 HL-60细胞48 h 和72 h 与空白对照相比均能下调HL-60细胞 VEGF mRNA 的表达及 VEGF 蛋白的分泌(P<0.01),三个不同浓度 DADS 组间差异均有统计学意义(P<0.01),其下调 HL-60细胞 VEGF mRNA 的表达及 VEGF 蛋白的分泌效果与药物浓度呈相关性(r>0.9,P<0.01)。结论 DADS 能明显抑制 HL-60细胞的增殖;DADS 可能通过抑制 HL-60细胞 VEGF mRNA 的表达及 VEGF 蛋白分泌发挥抗白血病的效应。
Objective To investigate the effects of diallyl disulfide (DADS) on the growth of leukemia cell line HL-60 and the expression of vascular endothelial growth factor (VFGF) mRNA and VEGF protein in HL-60 cells. Methods MTT assay was used to observe the inhibitory effect of DADS on the growth of HL-60 cells cultured in vitro. The content of VEGF protein in HL-60 cell culture supernatant before and after drug treatment was detected by ELISA. The semi-quantitative RT-PCR -60 cells VEGF mRNA expression levels. Results DADS had obvious growth inhibitory effect on HL-60 cells. The cell growth inhibition rates of HL-60 cells treated with 0.625, 1.250 and 2.500 μg / ml DADS for 24 h were (8.19 ± 3.34)% and (16.79 ± 2.07)%, respectively , (21.30 ± 2.72)%, respectively. The cell growth inhibition rates at 48 h were (11.93 ± 3.93)% (22.81 ± 2.31)% and (30.74 ± 2.03)%, respectively. 16.68 ± 2.37%, (28.54 ± 3.26)% and (36.59 ± 2.37)%, respectively. There was a significant relationship between the inhibitory rate and drug concentration of HL-60 cells (r> 0.9, P <0.01) Compared with the blank control, HL-60 cells could down-regulate the expression of VEGF in HL-60 cells at 48 h and 72 h after 0.625, 1.250, and 2.500 μg / ml DADS treatment respectively (r> 0.7, P <0.01) (P <0.01). There were significant differences among the three different concentrations of DADS (P <0.01), which down-regulated the expression of VEGF mRNA and the secretion of VEGF protein in HL-60 cells Drug concentration was correlated (r> 0.9, P <0.01). Conclusions DADS can significantly inhibit the proliferation of HL-60 cells. DADS may play an anti-leukemia effect by inhibiting the expression of VEGF mRNA and VEGF protein in HL-60 cells.