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目的 研究硫酸镁对缺血 再灌流心肌损伤的治疗作用 ,探讨更有效减轻再灌流心肌损伤的治疗措施。方法 采用Langendorff离体大鼠心脏灌流模型 ,心脏缺血 (停灌 ) 30min、再灌流 12 0min ,2 4只大鼠随机分成 3组 ,A组 :给Krebs-Henseleix (NKH)灌注液为对照组 ;B组 :再灌流开始至结束给予硫酸镁 ( 1 5mM)治疗 ;C组 :缺血前 5min及再灌流全程给予硫酸镁( 1 5mM)治疗。结果 B组、C组心脏功能 (LV +dp/dtmax,LV -dp/dtmax,LVDP)恢复显著优于A组 (P <0 0 5 ) ;C组再灌流后 2h心肌梗死范围减小 ,C组为 ( 2 8 75± 5 0 1) %、A组为( 38 98± 9 0 8) % ,P <0 0 5 ;C组肌酸激酶 (CK)活性增加 ,C组为 ( 1 0 1± 0 31)IU/mg蛋白、A组为 ( 0 75± 0 0 9)IU/mg蛋白 ,P <0 0 5 ;此外心律失常发生C组亦明显减少 ,C组 ( 0 81±0 5 3)次、A组 ( 2 31± 1 5 5 )次 ,P <0 0 5。结论 硫酸镁对缺血 再灌流损伤有保护作用 ,并与给药时间密切相关
Objective To study the therapeutic effect of magnesium sulfate on myocardial injury induced by ischemia-reperfusion injury and to explore the therapeutic measures to reduce myocardial damage after reperfusion. Methods Langendorff rat heart perfusion model was used. The hearts were ischemia for 30 min and reperfusion for 12 min. 24 rats were randomly divided into 3 groups. Group A: Krebs-Henseleix (NKH) Group B: magnesium sulfate (15mM) was given from the beginning to the end of reperfusion; Group C: magnesium sulfate (15mM) was administered 5 min before ischemia and throughout the reperfusion period. Results The recovery of cardiac function (LV + dp / dtmax, LV-dp / dtmax, LVDP) in group B and group C was significantly better than that in group A (P <0 05) Group A was (38 98 ± 90 8)%, P 0 05; the activity of creatine kinase (CK) in group C was increased, while in group C it was (101 ± 0 31) IU / mg protein in group A was (0 75 ± 0 0 9) IU / mg protein, P <0 05. In addition, the incidence of arrhythmia was also significantly decreased in group C ) Times, A group (2311 ± 155) times, P <0 05. Conclusion Magnesium sulfate has a protective effect on ischemia-reperfusion injury and is closely related to the administration time