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目的检测甲苯进入机体后对主要脏器(脑、心、肝、肺、肾脏)微量元素(Fe、En、Cu)的影响,探讨微量元素在甲苯的毒性方面作用机制。方法模拟环境甲苯污染途径,采用吸入染毒方法对小鼠进行染毒30 d后处死,取出脑、心、肝、肺、肾脏组织,测定小鼠各脏器的脏器系数,经消化后进行微量元素的测定。结果甲苯染毒组肝脏、肺脏和脑组织的脏器系数随着染毒剂量的增高出现下降趋势,并且各组之间差异有统计学意义(P<0.05);甲苯使肝脏、脑、肾脏中Fe、Cu含量均降低(P<0.05),而心脏和肺脏中2种元素高于对照组(P<0.05);以上差异均有统计学意义。Zn元素主要表现为肝脏和肾脏中减少(P<0.05),肺中含量增高(P<0.05);以上差异均有统计学意义。结论甲苯引起小鼠体内微量元素Zn、Cu、Fe的重分布,对肝脏、肺脏和脑组织产生一定的损伤。
Objective To investigate the effect of toluene on the contents of trace elements (Fe, En, Cu) in major organs (brain, heart, liver, lung and kidney) after entering the body and to explore the mechanism of action of trace elements on the toxicity of toluene. Methods The pathways of toluene pollution in the environment were simulated. The mice were sacrificed 30 days after inhalation exposure, and the brain, heart, liver, lung and kidney tissues were removed and the organ coefficients of each organ in mice were determined. After digestion, Determination of trace elements. Results The organ coefficients of liver, lung and brain in toluene-treated group showed a downward trend with the increase of exposure dose, and there was a significant difference between the groups (P <0.05). Toluene induced liver, brain and kidney Fe and Cu decreased (P <0.05), but two elements in heart and lung were higher than those in control group (P <0.05). The above differences were statistically significant. The contents of Zn in the liver and kidney were decreased (P <0.05), and the contents of Zn in the lung were increased (P <0.05). The above differences were statistically significant. Conclusion Toluene causes redistribution of trace elements Zn, Cu and Fe in mice, causing some damage to liver, lung and brain.