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目的:建立HPLC同时测定无梗五加[Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem.]果实中金丝桃苷和槲皮素-4’-O-β-D-半乳糖苷含量的方法。方法:采用TopsilTM(拓谱)C18(5μm,4.6 mm×250 mm)色谱柱,流动相为乙腈-0.2%乙酸水溶液(体积比为17.5∶82.5),流速1.0 mL.min-1,检测波长360 nm。结果:金丝桃苷的线性范围为0.036～0.356μg(r=0.9998),平均回收率为98.5%(RSD=2.1%),最低检测限为0.053μg.mL-1,最低定量限为0.178μg.mL-1;槲皮素-4’-O-β-D-半乳糖苷的线性范围为0.846～8.456μg(r=0.9995),平均回收率为99.5%(RSD=1.9%),最低检测限为0.126μg.mL-1,最低定量限为0.423μg.mL-1。供试品溶液48 h内稳定,金丝桃苷和槲皮素-4’-O-β-D-半乳糖苷峰面积的RSD分别为2.4%和2.6%。结论:方法可用于无梗五加果实的质量控制。
OBJECTIVE: To establish a method for the simultaneous determination of hyperin and quercetin -4’-O-β-D-galactoside in Acanthopanax sessiliflorus (Rupr.et Maxim.) Seem. METHODS: TopsilTM C18 (5μm, 4.6 mm × 250 mm) column was used. The mobile phase was acetonitrile-0.2% acetic acid solution (volume ratio was 17.5:82.5) and the flow rate was 1.0 mL.min-1. The detection wavelength was 360 nm. Results: The linear range of hyperoside was 0.036 ~ 0.356μg (r = 0.9998), the average recovery was 98.5% (RSD = 2.1%), the lowest detection limit was 0.053μg.mL-1 and the lowest limit of quantification was 0.178μg . The linear range of quercetin -4’-O-β-D-galactoside was 0.846 ~ 8.456μg (r = 0.9995) with the average recovery of 99.5% (RSD = 1.9% Limit of 0.126μg.mL-1, the lowest limit of quantification of 0.423μg.mL-1. The test solution was stable within 48 h. The RSDs of the peak areas of hyperin and quercetin -4’-O-β-D-galactoside were 2.4% and 2.6%, respectively. Conclusion: The method can be used for the quality control of Acanthopanax senticosus fruit.