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目的:研究石岐外感茶体外抗肠道病毒EV71的作用。方法:以利巴韦林为阳性对照药,观察药物对细胞的病变作用(CPE)影响,采用MTT法确定病毒感染后、病毒感染前和病毒、药物同时加入3种不同给药方法的50%细胞毒性浓度(TC50)、半数有效浓度(IC50)和治疗指数(TI)。结果:病毒感染后给药和病毒、药物同时加入法的IC50分别为0.77、0.33 mg/m L,TI为29.35、68.48,预防给药法未见明显的抑制病毒作用。结论:石岐外感茶在体外对肠道病毒EV71有抑制作用。
Objective: To study the effect of Shiqi exogenous tea against enterovirus EV71 in vitro. Methods: Ribavirin was used as a positive control drug to observe the effects of drugs on the cell pathogenicity (CPE). MTT assay was used to determine the effect of CPV on the cells. Before and after virus infection, 50% Cytotoxic concentration (TC50), half effective concentration (IC50) and therapeutic index (TI). Results: After administration of virus and infection of virus, IC50 of simultaneous addition of drugs were 0.77,0.33 mg / m L and TI was 29.35,68.48, respectively. There was no obvious inhibitory effect on the virus by prophylaxis. Conclusion: Shiqi exogenous tea can inhibit enterovirus EV71 in vitro.