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为明确我国长江流域和黄河流域主要植棉省落叶型菌系的致病力差异,选用4个抗性不同的陆地棉品种银瑞361、豫棉21、冀棉228和冀棉11作为鉴别品种,在温室可控条件下,采用苗期移苗蘸根接种法,对不同植棉省32个落叶型黄萎病菌的致病性进行了测定,并根据供试菌系在鉴别品种上病情指数进行聚类分析。苗期接种14 d后,抗、感品种间的病情指数差异较大,是落叶型菌系病情指数调查的合适时间。32个落叶型菌系致病力差异显著,可分为3个类型。第Ⅰ类型只有1个菌系,致病力弱;第Ⅱ类型菌系占供试菌系的62.5%,平均病情指数为36.8,致病力中等;第Ⅲ类型菌系占供试菌系34.4%,平均病情指数52.7,致病力较强,4个鉴别品种均表现感病。研究表明来源于不同植棉省菌系的平均致病力有一定差异,且来源于同一植棉省的落叶型菌系可归属于不同的致病类型。
In order to clarify the pathogenicity differences of the defoliated mycorrhizal fungi in the main cotton-growing provinces in the Yangtze River and Yellow River basins of China, four different upland cotton cultivars, Yinrui 361, Yumian 21, Hebei Jimian 228 and Hebei 11 were selected as the identification cultivars The pathogenicity of 32 defoliating Verticillium dahliae strains in different cotton planting provinces was determined under the controllable conditions in the greenhouse under the condition of seedling transplanting and dipping root inoculation. According to the disease index Cluster analysis. After 14 days of seedling inoculation, the disease index between the resistant and susceptible cultivars varied greatly, which was the suitable time for the investigation of the disease index of the deciduous fungi. The pathogenicity of 32 defoliating strains was significantly different and could be divided into three types. Type I only had one strain of bacteria, and the pathogenicity was weak. Type II strains accounted for 62.5% of the tested strains with an average disease index of 36.8 and medium pathogenicity. Type III strains accounted for 34.4 %, The average disease index 52.7, strong virulence, four identified varieties are susceptible. Studies have shown that the average virulence derived from different cotton-growing provinces have some differences in pathogenicity, and the deciduous flora originating from the same cotton-planting province can be attributed to different pathogenic types.