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Two antimicrobial substances produced by Bacillus subtilis strain B11 were purified by boiling, DEAE 52 anion exchangechromatography and aluminum oxide adsorption chromatography. These purified substances showed only one spot onsilica gel thin layer chromatography (TLC). Antimicrobial assays showed that antimicrobial substances A and B inhibitedthe growth of plant pathogens such as Fusarium oxysporum Schl f.sp. niveum, Rhizoctonia solani, Ralstonia solanacearumand Xanthomonas oryzae pv. oryzae. In addition, antimicrobial substance B could also inhibit the growth of Magnaporthegrisea. These two antimicrobial substances were resistant to proteolytic enzymes and temperature as high as 121°C.
Two antimicrobial substances produced by Bacillus subtilis strain B11 were purified by boiling, DEAE 52 anion exchange chromatography and aluminum oxide adsorption chromatography. These purified substances showed only one spot onsilica gel thin layer chromatography (TLC). Antimicrobial assays showed that antimicrobial substances A and B inhibitedthe growth of plant pathogens such as Fusarium oxysporum Schl f. sp. niveum, Rhizoctonia solani, Ralstonia solanacearumand Xanthomonas oryzae pv. oryzae. In addition, antimicrobial substance B could also inhibit the growth of Magnaporthe grisea. These two antimicrobial substances were resistant to proteolytic enzymes and temperature as high as 121 ° C.