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In this study,a natural cotton thread immunoassay device combined with gold nanorod(GNR) reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle(GNP) report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry(ASV) testing,which meet the requirement for clinical diagnosis.
In this study, a natural cotton thread immunoassay device combined with gold nanorod (GNR) reporter probe is developed for the rapid, sensitive and quantitative electrochemical determination of human ferritin, a lung cancer related biomarker. Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device. Enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle (GNP) report probe. device capable of measuring 1.58 ng / mL ferritin in 30 min by anodic stripping voltammetry (ASV) testing, which meet the requirement for clinical diagnosis.