论文部分内容阅读
目的探讨腺苷脱氨酶(ADA)和干扰素-γ(IFN-γ)在鉴别结核性胸腔积液及恶性胸腔积液中的应用价值。方法选取112例胸腔积液患者,其中结核性胸腔积液患者(结核性胸腔积液组)62例,恶性胸腔积液患者(恶性胸腔积液组)50例。应用酶速率法检测两组患者的胸腔积液中ADA的活性,同时用酶联免疫吸附法(ELISA)检测以上患者胸腔积液中IFN-γ浓度,比较两组患者中ADA活性及IFN-γ浓度的差异。结果结核性胸腔积液组中ADA的活性(50.5±9.4)U/L高于恶性胸腔积液组中ADA的活性(19.8±4.2)U/L,差异有统计学意义(P<0.05)。结核性胸腔积液组中IFN-γ的浓度(108.5±43.8)ng/L高于恶性胸腔积液组中IFN-γ的浓度(24.3±6.1)ng/L,差异有统计学意义(P<0.05)。结论 ADA和IFN-γ在鉴别结核性胸腔积液及恶性胸腔积液中均具有较高的诊断价值,二者联合亦可提高实验室检查的诊断阳性率,值得临床推广。
Objective To investigate the value of adenosine deaminase (ADA) and interferon-γ (IFN-γ) in the differential diagnosis of tuberculous pleural effusion and malignant pleural effusion. Methods One hundred and twelve patients with pleural effusion were selected, including 62 tuberculous pleural effusion patients (tuberculous pleural effusion group) and 50 malignant pleural effusion patients (malignant pleural effusion group). Enzyme-linked immunosorbent assay was used to detect ADA activity in pleural effusion in both groups. Meanwhile, the concentration of IFN-γ in pleural effusion was detected by enzyme-linked immunosorbent assay (ELISA). ADA activity and IFN-γ Differences in concentration. Results The ADA activity (50.5 ± 9.4) U / L in tuberculous pleural effusion group was higher than that in malignant pleural effusion group (19.8 ± 4.2) U / L, the difference was statistically significant (P <0.05). The concentration of IFN-γin tuberculous pleural effusion group (108.5 ± 43.8) ng / L was higher than that in malignant pleural effusion group (24.3 ± 6.1) ng / L, the difference was statistically significant (P < 0.05). Conclusion Both ADA and IFN-γ have high diagnostic value in the differential diagnosis of tuberculous pleural effusion and malignant pleural effusion. The combination of both can also increase the diagnostic positive rate of laboratory tests, which is worthy of clinical promotion.