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目前国外学者对正常婴儿的血浆ET进行了测试,但结果并不完全相同。为了今后临床工作中ET的进一步研究,我们测定了新疆乌鲁木齐市正常60例维吾尔族和汉族的婴儿血浆ET值,现报告如下。 对象和方法 一、对象:新疆医学院儿保门诊正常体检的婴儿共计60例(男30,女30),维吾尔族30例、汉族30例,年龄(1~12月)平均5.1±2.8月,1~3月者20例,4~12月者40例。 二、方法:清晨空腹取外周静脉血1.5ml,置于含10%EDTA-Na_20μl,抑肽酶400IU的试管中充分混匀,3000rpm离心10分钟,分离血浆,-20℃低温贮存待测。用放射免疫法测定。 三、ET试剂盒:由北京解放军总医院东亚免疫技术研究所提供。按说明书操作。 四、仪器:西安262厂生产的2008/P自动γ计数仪。
At present, foreign scholars have tested the plasma ET of normal infants, but the results are not exactly the same. In order to further study of ET in clinical work, we measured the ET value of 60 normal Uighur and Han Chinese infants in Urumqi, Xinjiang, and the report is as follows. Subjects and Methods I. Subjects: A total of 60 infants (30 men and 30 women) undergoing regular physical examinations in Xinjiang Medical College, 30 in Uyghur ethnicity and 30 in Han nationality, mean age (1-12 months) were 5.1 ± 2.8 months, 1 to 3 months in 20 cases, 4 to December in 40 cases. Second, the method: fasting peripheral blood taken 1.5ml, placed in 10% EDTA-Na_20μl, aprotinin 400IU in a tube thoroughly mixed, centrifuged at 3000rpm 10 minutes, the plasma was separated and stored at -20 ℃ to be tested. Measured by radioimmunoassay. Third, ET kits: provided by the East Asian Institute of Immunization Technology, Beijing People’s Liberation Army General Hospital. Follow the instructions. Fourth, equipment: Xi’an 262 Factory production of 2008 / P automatic γ counter.