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目的:对VEGF-D及其受体VEGFR-3在良性疾病肺组织和非小细胞肺癌(NSCLC)组织中的表达进行研究,探寻其相互之间的关系,从而进一步探讨VEGF-D、VEGFR-3在肺癌发生及NSCLC淋巴管生成和淋巴结早期转移中的作用。方法:收集非小细胞肺癌患者术后肺组织标本52例,良性疾病肺组织30例,应用半定量逆转录聚合酶链反应法(RT-PCR)检测良性疾病肺组织及肺癌组织中VEGF-D、VEGFR-3 mRNA的表达量。结果:VEGF-D mRNA、VEGFR-3 mRNA在肺癌组织中的表达水平较良性疾病肺组织表达水平明显升高(P<0.05)。VEGF-D、VEGFR-3 mRNA在肺癌组织中的表达与淋巴结转移无显著相关。结论:VEGF-D、VEGFR-3 mRNA的表达与肺癌发生有显著的相关性。
OBJECTIVE: To investigate the expression of VEGF-D and its receptor VEGFR-3 in benign disease lung tissues and non-small cell lung cancer (NSCLC) tissues and to explore the relationship between VEGF-D and VEGFR-3 in order to further explore the relationship between VEGF-D and VEGFR- 3 in lung cancer and NSCLC lymphangiogenesis and early lymph node metastasis role. Methods: Fifty-two patients with non-small cell lung cancer (NSCLC) and 30 patients with benign disease were collected. The expression of VEGF-D in lung tissue and lung cancer tissues was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) , The expression of VEGFR-3 mRNA. Results: The expression levels of VEGF-D mRNA and VEGFR-3 mRNA in lung cancer tissues were significantly higher than those in benign diseases (P <0.05). The expression of VEGF-D and VEGFR-3 mRNA in lung cancer tissues had no significant correlation with lymph node metastasis. Conclusion: The expression of VEGF-D and VEGFR-3 mRNA has a significant correlation with the occurrence of lung cancer.