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目的优化大鼠脊髓髓内胶质瘤模型的构建方法,并对模型进行评估。方法 Fischer大鼠髓内注射9L胶质瘤细胞悬液,对肿瘤细胞的植入量、植入部位、植入深度等进行筛选优化,根据筛选好的指标构建大鼠脊髓髓内胶质瘤模型,采用BBB(Basso,Beattie,and Bresnahan)评分量表评估大鼠下肢神经功能,采用高分辨率MRI检查及病理免疫组化方法检查模型大鼠肿瘤形成情况,评估模型构建效果。结果大鼠脊髓内植入9L胶质瘤细胞悬液构建大鼠髓内胶质瘤模型的优化指标为:植入平面在脊柱T10水平,植入量为6μL(1.0×105/mL)细胞悬液,植入位置在硬脊膜平面下3mm。植入9L胶质瘤细胞后2周左右大鼠出现明显的下肢神经功能障碍。影像学和病理学检查证实髓内有肿瘤细胞生长。结论成功构建大鼠髓内胶质瘤模型,为后续研究奠定了基础。
Objective To optimize the method of constructing spinal cord intramedullary glioma model and to evaluate the model. Methods Nineteen glioma cell suspensions were injected intramedullaryly into Fischer rats to screen and optimize tumor implantation, implantation site and implantation depth. According to the screened index, we constructed the intramedullary glioma model The BBB (Basso, Beattie, and Bresnahan) scale was used to evaluate the neurological function of the lower extremities of rats. High-resolution MRI and pathological immunohistochemistry were used to examine the tumor formation of the model rats. Results The optimized index of rat intramedullary glioma model with 9L glioma cells implanted into rat spinal cord was as follows: the level of implantation plane was at the level of the spine T10, and the implantation volume was 6μL (1.0 × 105 / mL) Liquid, implanted in the dura mater 3mm. After 2 weeks implantation of 9L glioma cells, rats showed obvious lower extremity nerve dysfunction. Imaging and pathological examination confirmed the presence of tumor cells in the marrow. Conclusion Successful establishment of intramedullary glioma model in rats will lay the foundation for subsequent research.