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目的：研究川芎中川芎嗪和阿魏酸的毛细管电泳分离分析的可行性。方法：通过用毛细管区带电泳分离、紫外检测模式研究内标、电泳缓冲液、进样方式、分离电压等对样品中川芎嗪和阿魏酸分离与定量的影响，优化实验条件。结果：以对硝基苯甲酸为内标，未涂层熔融石英毛细管（３９５ ｃ ｍ ×５０ μｍ Ｉ Ｄ，有效分离长度３４８ ｃｍ ） 为分离通道，３０ ｍ ｍｏｌ· Ｌ－ １ 硼砂缓冲液（ｐ Ｈ９４３） 为电泳介质，３４ ｋ Ｐａ·ｓ 压力进样，１７ ｋ Ｖ 分离电压，２９５ ｎｍ 检测，川芎嗪和阿魏酸分别在７ ～４２３ μｇ·ｍ Ｌ－ １ 和４ ～９００ μｇ·ｍ Ｌ－ １ 范围内可进行定量检测，回收率分别为１００９ ％ ±１９ ％ 和９９８ ％ ±１０ ％ 。结论：毛细管电泳法可用于川芎中川芎嗪和阿魏酸的同时分离分析。
Objective: To study the feasibility of separation and analysis of ligustrazine and ferulic acid in Chuanxiong by capillary electrophoresis. Methods: The separation and quantification of ligustrazine and ferulic acid in samples were studied by capillary zone electrophoresis separation and UV detection mode, and the experimental conditions were optimized. Results: With p-nitrobenzoic acid as an internal standard, uncoated fused silica capillary (395 μm × 50 μm ID, effective separation length of 348 cm) was used as the separation channel and 30 μmol·L -1 borax The buffer solution (p H9 43) was electrophoresed at 34 kPa · s pressure and 17 k V separation voltage at 295 nm. The concentrations of tetramethylpyrazine and ferulic acid were in the ranges of 7 ~ 423 μg · mL -1 and 4 ~ 900 μg · m L -1, the recoveries were 1009% ± 19% and 998% ± 10%, respectively. Conclusion: Capillary electrophoresis can be used for the simultaneous separation and analysis of ligustrazine and ferulic acid in Chuanxiong.