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目的探讨甲磺酸伊马替尼对放射性肺炎小鼠肺部氧化应激指标及转化生长因子-β1(TGF-β1)表达的影响。方法将45只清洁级C57BL/6小鼠随机平均分为空白组、治疗组和模型组。每天给予治疗组与模型组胸部照射,照射结束4 h后给予治疗组小鼠甲磺酸伊马替尼(给药剂量0.081 g/kg);其余组仅给予生理盐水。实验30d后,将各组小鼠处死,取其左右肺,分别采用HE染色及免疫组化观察其病理变化及肺部TGF-β1的表达情况。制作肺匀浆,检测其中谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、总抗氧化能力(T-Aoc)及超氧化物歧化酶(SOD)等氧化应激指标含量。结果治疗组GSH-PX、T-Aoc及SOD分别为(173.15±12.21)U、(119.33±11.06)U/mgprot及(1.73±0.33)U/mgprot,均明显高于模型组(P均<0.05),而MDA含量则为(0.68±0.08)nmol/mgprot,明显低于模型组(P<0.05)。HE染色及免疫组化结果显示,治疗组仅出现轻度肺泡炎改变及部分TGF-β1阳性表达,模型组则多为中级以上肺泡炎及高水平TGF-β1阳性表达,治疗组HE染色及免疫组化评分分别为(1.26±0.12)分和(0.31±0.12)分,均明显低于模型组(P<0.05)。结论甲磺酸伊马替尼可有效改善放射性肺炎小鼠肺部氧化应激紊乱,抑制小鼠肺部TGF-β1的表达,值得开展深入研究。
Objective To investigate the effects of imatinib mesylate on lung oxidative stress and the expression of transforming growth factor-β1 (TGF-β1) in mice with radiation pneumonitis. Methods 45 clean-grade C57BL / 6 mice were randomly divided into blank group, treatment group and model group. Mice in the treatment group and the model group were given thoracic radiation every day. The mice in the treatment group were treated with imatinib mesylate (dosage: 0.081 g / kg) 4 h after the irradiation, and the rest were given normal saline only. After 30 days of experiment, the mice in each group were sacrificed and their left and right lungs were sacrificed. The pathological changes and the expression of TGF-β1 in lung tissue were observed by HE staining and immunohistochemistry respectively. The lung homogenate was prepared and the oxidative stress such as glutathione peroxidase (GSH-PX), malondialdehyde (MDA), total antioxidant capacity (T-Aoc) and superoxide dismutase (SOD) Indicator content. Results The levels of GSH-PX, T-Aoc and SOD were (173.15 ± 12.21) U, (119.33 ± 11.06) U / mgprot and (1.73 ± 0.33) U / mgprot in the treatment group ), While the content of MDA was (0.68 ± 0.08) nmol / mgprot, which was significantly lower than the model group (P <0.05). The results of HE staining and immunohistochemistry showed that there was only slight alveolitis change and some TGF-β1 positive expression in the treatment group, while the expression of TGF-β1 was higher in the model group than in the moderate-grade alveolitis group. The HE staining and immunization in the treatment group The histological scores were (1.26 ± 0.12) points and (0.31 ± 0.12) points, respectively, which were significantly lower than those in the model group (P <0.05). Conclusion Imatinib mesylate can effectively improve the pulmonary oxidative stress disorder and inhibit the expression of TGF-β1 in the lungs of mice with radiation pneumonitis, which deserves further study.