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以大豆7S蛋白β亚基基因为靶基因(基因编号:AB030840),利用RT-PCR克隆得到大豆7S蛋白β亚基基因核心序列398 bp,构建了以抗除草剂基因BAR为筛选标记的安全植物RNAi表达载体BAR-7αp-β。分子生物学检测表明7S蛋白β亚基基因的表达载体构建成功。研究结果为应用RNA干扰技术降低大豆过敏原,提高大豆蛋白品质奠定了基础。
The core sequence of soybean 7S protein β subunit gene was cloned by RT-PCR with the target gene of soybean 7S protein β subunit gene (gene number: AB030840) as 398 bp, and the safety plants with herbicide resistant gene BAR as screening marker RNAi expression vector BAR-7αp-β. Molecular biology tests showed that the expression vector of 7S protein β subunit gene was successfully constructed. The results laid the foundation for the application of RNAi technology to reduce soybean allergens and improve the quality of soy protein.