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目的 研究虎杖苷对卵巢摘除骨质疏松大鼠β-Catenin及GSK-3β蛋白表达的影响,探索其对卵巢摘除骨质疏松大鼠的保护作用及可能的作用机制.方法 SPF级♀大鼠60只,随机分为假手术组、模型组、虎杖苷低(5 mg·kg-1.d-1)、中(15mg·kg-1.d-1)、高剂量组(45 mg.kg-1 ·d-1),每组12只,灌胃给药.除假手术组外,其余各组采用摘除大鼠双侧卵巢的方法模拟骨质疏松模型.12周后,用双能X线吸收法测量骨密度(bone mineral density,BMD),ELISA法检测E2、ALP、TRAP等生化指标的水平;Western blotting法检测β-Catenin和GSK-3β蛋白的表达,qRTPCR法检测β-Catenin和GSK-3βmRNA的表达.结果 与模型组比较,虎杖苷各组BMD,E2显著升高(P＜0.01)、血清ALP、TRAP显著降低(P＜0.01);β-Catenin蛋白的表达升高(P＜0.01),GSK-3β表达降低(P＜0.01);β-Catenin mRNA表达显著升高(P＜0.01),GSK-3β mRNA表达显著降低(P＜0.01).结论 虎杖苷对去卵巢骨质疏松有一定的治疗作用,其机制可能与增加β-Catenin表达,减少GSK-3β表达有关.“,”OBJECTIVE To study the effect of polydatin(PD) on the expression of β-Catenin and GSK-3β protein in ovariectomy osteoporosis rats.METHODS The 60 female SPF rats were randomly divided into sham operation group,model group,PD low(5 mg·kg-1·d-1),middle(15 mg·kg-1 ·d-1) and high dose(45 mg·kg-kd-1) groups (12 rats in each group,ig),every group were established by ovariectomy except sham group.After 12 weeks,the bone mineral density (BMD) of these rats was detected by dual energy X-ray absorptionmetry,ELISA method was used to detect serum estradiol(E2),bone alkaline phosphatase(ALP),tartrate resistant acid phosphatase(TRAP) and other biochemical indicators of the level;Western blotting was used to detect the expression of β-Catenin and GSK-3β protein;qRTPCR was used to detect the expression of β-Catenin and GSK-3β mRNA.RESULTS Compared with model group,PD groups increased BMD,E2 significantly(P＜0.01),decreased ALP,TRAP level(P＜0.01) and increased the expression of β-Catenin protein and mRNA(P＜0.01);decreased the expression of GSK-3β protein and mRNA (P＜0.01).CONCLUSION PD has therapy effects in ovariectomy osteoporosis rats.Its mechanism could be relative with increasing the expression of β-Catenin protein and decreasing expression of GSK-3β.