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目的:研究NDV7793序贯5-FU治疗方案对小鼠结肠癌肝转移的抑制效果,并初步探讨序贯联合用药对荷瘤小鼠免疫功能的影响。方法:采用脾内注射法建立小鼠结肠癌肝转移模型;将小鼠模型随机分为3组:PBS阴性对照组(0.1 m L/d,10 d)、NDV7793组[512 HU/(kg·d),5 d]和NDV7793序贯5-FU组[512 HU/(kg·d),5 d+10 mg/(kg·d),5 d]。根据受试小鼠生存时间、体重变化、肝脏转移癌灶数、肝重等指标评价序贯用药效果;根据受试小鼠胸腺指数及肝脏IFN-γ的量初步观察序贯用药对荷瘤小鼠免疫功能的影响。结果:小鼠生存时间序贯给药组(32 d)长于NDV7793单独给药组(30 d)及PBS阴性对照组(17d)(P<0.05);转移癌结节序贯给药组(30.60±9.32)少于PBS阴性对照组(273.30±30.73)(P<0.05),但与NDV7793组(24.83±6.90)相比,两者均无统计学差异(P>0.05),序贯给药组小鼠肝重轻于PBS阴性对照组(P<0.05);序贯给药组的胸腺指数低于NDV7793组(P<0.05),但其IFN-γ表达水平远高于NDV7793组,差异具有统计学意义(P<0.05)。结论:NDV7793序贯5-FU对小鼠结肠癌肝转移具有抑制效果;与NDV7793单独用药相比,序贯联合用药对荷瘤小鼠生存期的延长及免疫功能的激活,均有一定的提高。
OBJECTIVE: To study the inhibitory effect of NDV7793 sequential 5-FU treatment on hepatic metastasis of colon cancer in mice and to investigate the effect of sequential combination therapy on immune function of tumor-bearing mice. Methods: The model of hepatic metastasis of mouse colon cancer was established by intraperitoneal injection. The mice were randomly divided into 3 groups: PBS negative control group (0.1 m L / d, 10 d), NDV7793 group [512 HU / (kg · d), 5 d] and NDV7793 sequential 5-FU group [512 HU / (kg · d), 5 d + 10 mg / (kg · d), 5 d]. According to the survival time of mice, body weight changes, the number of liver metastases, liver weight and other indicators to evaluate sequential effect; according to the thymus index of mice and the amount of liver IFN-γ preliminary observation of sequential treatment of tumor-bearing small Impact of rat immune function. Results: The survival time of mice in the sequential treatment group (32 d) was longer than that of NDV7793 alone group (30 d) and PBS negative control group (17 d) (P <0.05) ± 9.32) was lower than PBS negative control group (273.30 ± 30.73) (P <0.05), but no significant difference was found between NDV7793 group (24.83 ± 6.90) and control group (P> 0.05) (P <0.05). The thymus index of sequential administration group was lower than that of NDV7793 group (P <0.05), but the level of IFN-γ was much higher than that of NDV7793 group Significance (P <0.05). Conclusion: Sequential 5-FU of NDV7793 can inhibit the hepatic metastasis of colon cancer in mice. Compared with NDV7793 alone, sequential combination therapy can prolong survival and activate immune function of tumor-bearing mice .