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目的:观察康肺扶正方对肺癌荷瘤小鼠瘤组织的抑制作用及对DNA甲基转移酶1、3β(DNMT1、DNMT3β)、增殖细胞核抗原(PCNA)、白细胞介素6(IL-6)、白细胞介素10(IL-10)、干扰素-γ(INF-γ)等血清指标影响。方法:以人肺癌细胞的裸鼠荷瘤鼠模型作为研究对象,将24只裸鼠荷瘤鼠随机分成4组,分别为模型组,环磷酰胺(CTX)组,康肺扶正方小、大剂量组。模型组给予生理盐水0.4ml/d灌胃;环磷酰胺组予环磷酰胺(20mg·kg~(-1)·d~(-1))腹腔注射,1次/d;康肺扶正方小、大剂量组分别给予不同剂量的康肺扶正方灌胃,均1次/d,连续4周。检测各组裸鼠肿瘤体积、抑瘤率;且用免疫组织化学方法检测DNMT1、DNMT3β、PCNA表达;用酶联免疫吸附试验盒(ELISA)检测IL-6、IL-10、INF-γ。结果:与模型组相比,小剂量康肺扶正方组的瘤体积有所缩小,但未达到统计学意义;大剂量康肺扶正方组的瘤体积缩小,差异有统计学意义。大剂量康肺扶正方组DNMT1、DNMT3β、PCNA阳性表达率明显减低(P<0.05)。大剂量康肺扶正方小鼠血清中IL-6、IL-10、INF-γ较其他组升高,差异有统计学意义(P<0.05)。结论:康肺扶正方的体内抗癌机制可能部分通过抑制肿瘤的增殖活性而实现。
Objective: To observe the inhibitory effect of Kangfeifuzheng Recipe on the tumor of lung cancer-bearing mice and its effect on the expression of DNMT1, DNMT3β, PCNA, IL-6, , Interleukin 10 (IL-10), interferon-γ (INF-γ) and other serum indicators. Methods: Twenty-four nude mice bearing tumor-bearing mice model were randomly divided into four groups: model group, cyclophosphamide (CTX) group, Kangfu Fangzhengfang small, large Dose group. Rats in the model group were given 0.4 ml / d normal saline, while those in the cyclophosphamide group were injected intraperitoneally with cyclophosphamide (20 mg · kg -1 · d -1) once per day. , High-dose group were given different doses of Kangfeizhu square gavage, were 1 / d, for 4 weeks. The tumor volume and tumor inhibition rate of the nude mice in each group were detected. The expressions of DNMT1, DNMT3β and PCNA were detected by immunohistochemistry. The levels of IL-6, IL-10 and INF-γ were detected by enzyme-linked immunosorbent assay (ELISA) Results: Compared with the model group, the tumor volume of Kangfushengzhengfang in the low dose group contracted but did not reach the statistical significance. The tumor volume of the large dose Kangfushengzhengfangfang group was reduced, the difference was statistically significant. The positive expression rates of DNMT1, DNMT3β, PCNA in high dose Kangfeifu square group were significantly decreased (P <0.05). The levels of IL-6, IL-10 and INF-γ in the serum of high-dose Kangfeifuzheng mice were significantly higher than those in other groups (P <0.05). Conclusion: The anti-cancer mechanism of Kangfeifangzhengfang may be partly achieved by inhibiting the proliferation activity of tumor.