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本研究对大豆Gm NFYB1(Glyma02g17310)进行基因功能和表达分析,利用Plant CARE分析启动子元件发现,在基因启动子序列中含有多个与ABA、抗旱、光反应相关的元件。在The Bio-Analytic Resource for Plant Biology数据库中分析了Gm NFYB1的同源基因At NFYB5在不同逆境胁迫和不同激素处理时的基因表达情况,表明该基因表达具有组织表达特异性,参与盐胁迫、渗透胁迫等反应。Gm NFYB1在大豆的q RT-PCR结果表明,该基因受ABA、Na Cl、PEG诱导上调表达,参与大豆抗逆反应。
In this study, gene function and expression analysis of soybean Gm NFYB1 (Glyma02g17310) was carried out. The plant CARE was used to analyze the promoter elements, which contained many elements related to ABA, drought resistance and light response in the gene promoter sequence. The gene expression of At NFYB5, a homologue of Gm NFYB1 under different stress conditions and different hormone treatments, was analyzed in The Bio-Analytic Resource for Plant Biology database, indicating that the gene has tissue-specific expression and is involved in salt stress and osmosis Stress and other reactions. Q RT-PCR results of Gm NFYB1 in soybean showed that the gene was up-regulated by ABA, NaCl and PEG, and was involved in soybean anti-retrogradation.