【摘 要】
：
AIM To establish an extracorporeal bioartificial liver support system (EBLSS) using cultured human liver cells and to study its support effect for fulminant hep
【机 构】
：
Clinical Center of Infectious Diseases
【基金项目】
：
The national key project fund
论文部分内容阅读
AIM To establish an extracorporeal bioartificial liver support system (EBLSS) using cultured human liver cells and to study its support effect for fulminant hepatic failure (FHF).METHODS The liver support experiment of EBLSS consisting of aggregates cultured human liver cells, hollow fiber bioreactor, and circulation unit was carried out in dizhepatic dogs.RESULTS The viability of isolated hepatocytes and nonparenchymal liver cells reached 96%. These cells were successfully cultured as multicellular spheroids with synthetic technique. The typical morphological appearance was retained up to the end of the artificial liver experiment. Compared with the control dogs treated with EBLSS without liver cells, the survival time of artificial liver support dogs was significantly prolonged. The changes of blood pressure, heart rate and ECG were slow. Both serum ammonia and lactate levels were significantly lowered at the 3rd h and 5th h. In addition, a good viability of human liver cells was noted after 5 h experiment.CONCLUSION EBLSS playing a metabolic role of cultured human hepatocytes, is capable of compensating the function of the liver, and could provide effective artificial liver support and therapy for patients with FHF.
其他文献
INTRODUCTIONImmune function status is corresponded to genesis and progress of tumor, and cell immune plays a major role in anti-tumor immunity. In recent years,
INTRODUCTIONThere have been many reports on cancer therapy with lymphokine-activated killer (LAK) cells and interleukin-2 (IL-2), but the proliferative response
AIM To evaluate the curative effect of pylorus and pyloric vagus-preserving gastrectomy (PPVPG) on peptic ulcer.METHODS Treating 132 cases of GU and DU with PPV
AIM To study the angiogenesis induced by liver cancer with different metastatic potentials using corneal micropocket model in nude mice.METHODS Corneal micropoc
A number of different methods have been used to estimate gastric emptying in humans, and all have their advantages and disadvantages. The method of choice will
Colon cancer has a high incidence in the world,especially in Western countries, and the incidence is also increasing in China in recent years.
AIM To investigate the relationship between alanyl-glutamine (ALA-GLN) and glutathione (GSH) biosynthesis in hepatic protection.METHODS Twenty male Wistar rats
INTRODUCTIONAspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity are usually used as biochemical criteria for the evaluation of liver le
INTRODUCTIONWe studied the recipient and graft pathophysiologic changes after transplantation, the inducement of immunotolerance, the regularity of chronic reje
AIM To study the inducible expression of hepatitis C virus ns3 gene (HCV ns3) in eukaryotic cells.METHODS The ns3 gene was obtained from plasmid pBns3 by polyme