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目的鉴定1株脑梗死患者血液中分离的生痰二氧化碳嗜纤维菌。方法用血培养仪对脑梗死患者静脉血标本进行需氧和厌氧培养,分离培养阳性培养物,用生化鉴定卡进行生化鉴定,用质谱分析及16S r RNA基因序列分析进行补充鉴定,并用KB法进行药敏试验。结果该菌为革兰阴性细梭状杆菌,常规的方法无法鉴定至种。结合质谱分析及16S r RNA基因序列分析结果,鉴定为生痰二氧化碳嗜纤维菌。该菌对阿米卡星、环丙沙星、红霉素、阿奇霉素抑菌环均为6 mm,对其他药物的抑菌环较大。结论质谱分析和16S r RNA基因序列分析为常规方法无法鉴定至种的细菌鉴定提供了可靠依据,将本例细菌鉴定为生痰二氧化碳嗜纤维菌。
Objective To identify the phlegm-producing carbon dioxide-producing phlegm in the blood of a patient with cerebral infarction. Methods Venous blood samples from patients with cerebral infarction were cultured aerobically and anaerobically in a blood culture instrument. Positive cultures were isolated and cultured, biochemically identified with biochemical identification cards, and identified by mass spectrometry and 16S rRNA gene sequence analysis. Act for susceptibility testing. The results of the bacteria Gram-negative Corynebacterium parvum, the conventional method can not identify to species. Combined with mass spectrometry analysis and 16S rRNA gene sequence analysis results, identified as phlegm carbon dioxide fiber-producing bacteria. The bacteria of amikacin, ciprofloxacin, erythromycin, azithromycin antibacterial ring are 6 mm, the larger inhibition of other drugs ring. Conclusion Mass spectrometry and 16S rRNA gene sequence analysis provide a reliable basis for the identification of the species of bacteria by conventional methods. The bacteria in this case were identified as Phytophthora capsici.