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脲酶电极是目前研究得较多的酶电极之一,为了使该酶电极能更好地适应临床检验的要求,并尽快将脲酶电极推广应用,我们对6种不同载体固定化方法、3种不同基础电极及不同测试条件对电极性能的影响作了系统研究。结果表明,当采用化学交联法将脲酶固定在尼龙网上,用氨气敏电极作基础电极时制得的脲酶电极性能最优,当选用 pH=8.0～8.5的磷酸盐缓冲液时,电极在25～37℃范围内可获得满意的效果,使用寿命可达3个月以上。在上述工作的基础上,结合现有的流动注射分析仪,我们设计了一个采用脲酶
Urease electrode is one of the most studied enzyme electrodes. In order to make the enzyme electrode better adapt to the requirements of clinical testing and to promote urease electrode as soon as possible, we have six kinds of different methods of immobilization of carrier, three kinds of different The influence of basic electrode and different test conditions on the performance of the electrode was systematically studied. The results showed that when urease was immobilized on nylon net by chemical cross-linking method, urease electrode prepared by ammonia gas sensing electrode had the best performance. When using phosphate buffer solution with pH = 8.0-8.5, 25 ~ 37 ℃ range to obtain satisfactory results, the service life of up to 3 months or more. Based on the above work, combined with the existing flow injection analyzer, we designed a urease