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目的:利用基因芯片技术对原发性宫颈癌标本进行分析,以期探讨宫颈癌基因组中存在的癌基因表达的变异,确定与宫颈癌相关的候选原癌或抑癌基因,为宫颈癌致病机理的研究提供新的线索和依据。方法:对原发性宫颈癌标本组织和正常对照组织进行mRNA抽提,分别给予荧光素Cy5和Cy3标记以制备cDNA探针,并与基因芯片进行杂交和分析。结果:在原发性宫颈癌标本组织中共筛选出存在表达差异的原癌或抑癌基因共11条,占候选基因的3.4%,其中表达明显下降的基因7条,上调基因4条。结论:运用基因芯片技术筛选出与宫颈癌相关的候选原癌或抑癌基因,为宫颈癌致病机理的研究提供新的线索和依据。
OBJECTIVE: To use gene chip technology to analyze primary cervical cancer specimens in order to explore the variation of oncogene expression in cervical cancer genome, to identify candidate proto-oncogenes or tumor suppressor genes related to cervical cancer, The research provides new clues and basis. Methods: mRNA was extracted from specimens of primary cervical cancer and normal control tissues, labeled with fluorescein Cy5 and Cy3, respectively, to prepare cDNA probes and hybridized and analyzed with cDNA microarray. Results: A total of 11 primary or tumor suppressor genes were found in primary cervical cancer tissues, accounting for 3.4% of the candidate genes. Among them, 7 were significantly decreased and 4 were up-regulated. CONCLUSIONS: Screening candidate proto-oncogenes or tumor suppressor genes associated with cervical cancer using gene chip technology will provide new clues and evidences for the study on the pathogenesis of cervical cancer.