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目的 探讨膀胱癌患者外周血中特异性细胞角蛋白 2 0 (CK 2 0 )mRNA在诊断中的意义。 方法 膀胱癌患者 91例 ,半巢式逆转录聚合酶链反应检测其外周血有核细胞成分中CK 2 0mRNA含量 ,2 5例健康人作为对照。 结果 91例患者中 37例 (41 % )外周血CK 2 0mRNA阳性表达 ,其中证实有转移的 2 0例中 1 7例 (85 % )阳性 ;对照组 2 5例均为阴性。外周血细胞CK 2 0mRNA的阳性率在有远处转移的病例中显著升高 (P <0 .0 1 )。 结论 检测膀胱癌患者外周血中CK 2 0mRNA对早期发现膀胱癌细胞的血行播散有重要参考价值
Objective To investigate the significance of specific cytokeratin 20 (CK 2 0) mRNA in peripheral blood of patients with bladder cancer. Methods 91 cases of bladder cancer were examined by semi-nested reverse transcriptase polymerase chain reaction (PCR-RT-PCR). The CK 2 0 mRNA levels in peripheral blood mononuclear cells were measured. Twenty-five healthy controls were used as controls. Results Thirty-seven patients (41%) had positive CK 2 0 mRNA in peripheral blood, of which 17 (85%) were positive in 20 cases confirmed as metastasis and 25 in control group. The positive rate of CK20 mRNA in peripheral blood cells was significantly higher in patients with distant metastasis (P <0.01). Conclusion Detection of CK 2 0 mRNA in peripheral blood of patients with bladder cancer has important reference value for early detection of hematogenous dissemination of bladder cancer cells