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背景:随着年龄的增加,脑内超氧化物歧化酶活性降低、丙二醛含量增加而造成的损伤作用越来越受到重视;猪脑肽溶液能否通过血脑屏障进入脑组织对脑神经起保护作用,目前只是临床病例报道,对其实验研究国、内外报道较少。目的:探讨猪脑肽溶液对D-半乳糖拟衰老小鼠脑以及肝脏组织中丙二醛活性和超氧化物歧化酶含量的影响。设计:以实验动物为研究对象的随机对照实验观察。单位:解放军总医院老年医学研究所。材料:实验于2001-02/2001-04在解放军总医院老年医学研究所完成(军队重点实验室)。选择NH小鼠82只,雌雄各半,解放军总医院医学实验动物中心(清洁级)提供,体质量18～22g,随机分为对照组、猪脑肽溶液(施普善)大、中、小剂量组、阿尼西坦组(阳性对照组)和D-半乳糖组。方法:各组分别给药,连续给6周,采用D-半乳糖诱导的脑老化小鼠动物模型。各组分别用化学发光法测定超氧化物歧化酶活性,用硫代巴比妥酸比色法测定MDA含量。主要观察指标:各组小鼠的SOD活性和丙二醛含量。结果:猪脑肽溶液大、中剂量均能显著提高脑以及肝脏组织中超氧化物歧化酶活性[分别为(30.23±5.23),(29.65±4.88);(19.84±5.79),(16.75±5.32)μkat/g],降低丙二醛含量[分别为(630.00±59.40),(626.74±49.68);(934.90±207.50),(916.06±235.31)nmol/g],与D-半乳糖组比较[分别为(22.89±4.54),(12.26±6.31)μkat/g;(749.69±92.80),(1212.89±142.54)nmol/g],差异均有显著性意义(P<0.05或P<0.01)。结论:猪脑肽溶液具有明显的抗氧化能力,对衰老小鼠脑神经细胞及肝细胞有一定的保护作用,为评估其对老年机体脑及肝细胞的抗自由基氧化损伤作用提供理论支持。
BACKGROUND: With the increase of age, the activity of SOD in the brain is decreased and the damage caused by the increase of malondialdehyde content is paid more and more attention. Whether pig brain peptide solution can penetrate the blood-brain barrier and enter the brain Play a protective role, currently only clinical cases reported, its experimental study of the country, less internal and external reports. Objective: To investigate the effect of porcine brain peptide solution on malondialdehyde (MDA) activity and superoxide dismutase (SOD) in brain and liver tissue of D-galactose-induced aging mice. Design: A randomized controlled trial of experimental animals. Unit: Institute of Geriatrics, PLA General Hospital. Materials: The experiment was performed at the Institute of Geriatrics, PLA General Hospital from February 2001 to April 2001 (Key Laboratory of the PLA Army). Eighty-two NH mice were chosen, male and female, respectively. The PLA General Hospital Medical Laboratory Animal Center (cleaning grade) was provided with body weight 18-22g and randomly divided into control group, pig brain peptide solution , Aniracetam group (positive control group) and D-galactose group. Methods: The rats in each group were given continuous administration for 6 weeks, and the animal model of brain aging was induced by D-galactose. The activity of superoxide dismutase (SOD) was determined by chemiluminescence method and MDA content by thiobarbituric acid colorimetry. MAIN OUTCOME MEASURES: SOD activity and malondialdehyde content of mice in each group. Results: The results showed that superoxide dismutase activity in brain and liver tissues were increased significantly (P <0.05), and the levels of superoxide dismutase in brain and liver tissues were significantly higher (P <0.01) μkat / g] and MDA content [(630.00 ± 59.40), (626.74 ± 49.68), (934.90 ± 207.50) and (916.06 ± 235.31) nmol / g, respectively (22.89 ± 4.54), (12.26 ± 6.31) μkat / g; (749.69 ± 92.80), (1212.89 ± 142.54) nmol / g, respectively. There was significant difference between the two groups (P <0.05 or P <0.01). CONCLUSION: Porcine brain peptide solution has obvious anti-oxidation ability, which can protect brain cells and hepatocytes of senile mice and provide theoretical support for the evaluation of anti-free radical oxidative damage in the brain and hepatocytes of the aged.