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以绿色荧光蛋白腺病毒重组体(Replication deficient adenovirus green fluorescence protein recombinant,AdCMV-GFP)为对照,用p53腺病毒重组体(Replication deficient adenovirus p53 recombinant,AdCMV-p53)转染经0、0.25、0.5、1.0、1.5和2.0Gyγ射线预辐射的HepG2(wtp53)、Hela(wtp53,wtP53低水平表达)和HT-29(mtp53,mtP53过表达)细胞,用克隆形成法检测肿瘤细胞存活,探讨AdCMV-p53转染对p53基因状态与功能不同肿瘤细胞辐射敏感性的影响。结果显示,AdCMV-p53转染不仅明显提高肿瘤细胞辐射敏感性,而且与肿瘤细胞内在p53基因状态与功能有关。
The recombinant adenovirus vector was transfected into AdCMV-p53 cells by using recombinant adenovirus vector (AdCMV-GFP) HepG2 (wtp53), Hela (wtp53, low wtP53 expression) and HT-29 (mtp53, mtP53 overexpression) cells were pre-irradiated with 1.0, 1.5 and 2.0Gy gamma rays. The survival of tumor cells was detected by clone formation assay. Effect of Transfection on Radiation Sensitivity of p53 Genes to Different Tumor Cells. The results showed that AdCMV-p53 transfection not only significantly increased the radiosensitivity of tumor cells, but also correlated with the status and function of p53 gene in tumor cells.