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目的:评价忍冬藤多糖的体内、体外抗氧化能力。方法:以热水回流提取法(料水比1∶25.61,89.46℃下提取4.53h,提取3次)及分段纯化得到的忍冬藤多糖为材料,通过体外DPPH清除率、总的抗氧化活性的测定及小鼠CCl4肝损伤的体内抗氧化实验来评价忍冬藤多糖的抗氧化能力。结果:忍冬藤多糖体外清除DPPH自由基、总的抗氧化活性均随多糖浓度的增加而上升;体内实验显示忍冬藤多糖可显著提高肝损伤小鼠血清和肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,降低丙二醛(MDA)的含量。结论:忍冬藤多糖具有较强的体内体外抗氧化能力。
Objective: To evaluate the in vivo and in vitro antioxidant capacity of Lonicera japonica polysaccharide. Methods: The extraction of hot water (ratio of material to water 1:25.61, extraction and extraction of 4.53h at 49.46 ℃ for 3 times) and purification of Grifola frondosa polysaccharide were used as materials. The DPPH clearance rate, total antioxidant activity And in vivo CCl4 liver injury in vivo antioxidant test to evaluate the antioxidant capacity of honeysuckle polysaccharide. Results: The DPPH radical scavenging activity of polysaccharide from H. lucidum in vitro increased with the increase of polysaccharide concentration in vivo. In vivo experiments showed that H. lucidum polysaccharide could significantly increase the activity of superoxide dismutase (SOD) in serum and liver of mice with liver injury, Glutathione peroxidase (GSH-Px) activity, reduce the content of malondialdehyde (MDA). Conclusion: The honeysuckle polysaccharide has a strong in vivo antioxidant capacity.