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[目的]了解感染家族成员中菌株的基因多态性,有助于明确Hp传播方式和宿主适应性。[方法]从家族中母亲胃窦、胃体和孩子们胃窦部活检标本中分离培养Hp,从每例菌株中分离出3个单克隆菌株。采用随机扩增多态性DNA指纹法(RAPD)检测Hp菌株的基因多态性。[结果]分离自孩子们的单克隆菌株的RAPD指纹图相同或十分相似,与母亲的菌株有同源性,而母亲自身感染的菌株具有多态性,提示混合感染。[结论]感染家族成员中Hp菌株的基因型相对均一。为进一步探明Hp的传播方式及其宿主适应性奠定了基础。
[Objective] To understand the genetic polymorphisms of the strains in the infected families and help to clarify the mode of Hp transmission and host adaptability. [Method] Hp was isolated and cultured from the gastric antrum of gastric mucosa in the antrum, gastric body and children. Three monoclonal strains were isolated from each of the strains. Random amplified polymorphic DNA fingerprinting (RAPD) was used to detect the genetic polymorphism of Hp strains. [Results] The RAPD fingerprints of the monoclonal isolates isolated from children were the same or very similar, had homology with the mother’s strain, while the mother’s own infected strains were polymorphic, suggesting mixed infection. [Conclusion] The genotypes of Hp strains in the infected family members were relatively uniform. Which laid the foundation for further ascertaining the mode of Hp transmission and its host adaptability.