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为明确金属元素在白背飞虱Sogatella furcifera(Horváth)体内的分布及其含量动态,将白背飞虱长翅成虫在实验室中用超纯水分别饲养0,24,48,96h后测量其体重变化;并用电感耦合等离子体质谱(inductively coupledplasma mass spectrometry,ICP-MS)检测各个处理虫体内12种金属元素(Cu,Zn,Cd,Mn,Ca,Fe,Na,As,U,Mg,K和Pb)的含量。以白背飞虱整个体重(W)、有机体组织重量(O)、体内可排泄内容物重量(E)及可排泄内容物排出体外的速率(r)为参数,构建其体重变化的修正指数模型Wt=O+E(1-r)t。基于白背飞虱体内金属元素总含量(WCt)为有机体组织内含量(OC)和可排泄内容物中含量(EC)按两者百分率(OPt和EPt)加权之和,即WCt=OC·OPt+EC·EPt,分别估计白背飞虱体内组织和可排泄内容物中金属元素含量。模拟分析结果表明:Cu,Zn,Cd,Mn和Ca这5种元素在白背飞虱组织内的含量显著高于其可排泄内容物中的含量(P<0.05),表明这些金属元素可被昆虫有机体组织吸收、积累,并稳定地存在于昆虫的有机体组织之中。Fe,Na,As,U,Mg,K和Pb这7种元素在白背飞虱有机体组织内的含量与其可排泄内容物中的含量接近,其在有机体组织中和可排泄内容物中的分布差异不显著(P>0.05),以相对平衡状态存在于白背飞虱有机体组织和可排泄内容物中。该分析方法可能为其他小型和微型昆虫体内金属元素的分布、积累、排泄的研究提供借鉴。
In order to clarify the distribution and content dynamics of metal elements in Sogatella furcifera (Horváth), the adult white-backed planthopper livers were kept in the laboratory for 0, 24, 48 and 96 hours respectively with ultrapure water and then measured Body weight was measured. The contents of 12 metal elements (Cu, Zn, Cd, Mn, Ca, Fe, Na, As, U, Mg and K) in each treatment group were determined by inductively coupled plasma mass spectrometry (ICP-MS) And Pb) content. The body weight index (W), body tissue weight (O), body excretion content (E) and excretion rate (r) of excreted contents were used as parameters to construct a modified exponential model Wt = O + E (1-r) t. Based on the total metal element content (WCt) in the body of the whitebacked planthopper, it is the sum of the weight (OCt) of the organism and the content of the excretable substance (EC) by the weight of both OPt and EPt, ie WCt = OC · OPt + EC · EPt, respectively, to estimate the contents of metal elements in the tissues and excretable contents of white-backed planthopper. The simulation results showed that the content of Cu, Zn, Cd, Mn and Ca in the white-backed planthopper was significantly higher than that in the excretable contents (P <0.05), indicating that these metal elements could be Organisms of insect tissues absorb, accumulate, and stably exist in the insect’s organism tissue. The contents of Fe, Na, As, U, Mg, K and Pb in the tissues of whitebacked planthopper were similar to those of excretable contents, and their contents in tissues and excretable contents The difference was not significant (P> 0.05), and existed in the tissue and excretable contents of the whitebacked planthopper in relative equilibrium. The analysis method may provide reference for the study of the distribution, accumulation and excretion of metal elements in other small and mini insects.