Construction of a Microsatellite-enriched Library of Rhododendron simsii Planch.

• 作 者: Shuzhen WANG Weibin JIN Jun XI
• 机 构: HubeiKeyLaboratoryofEconomicForestGermplasmImprovementandResourceComprehensiveUtilization/CollegeofL
• 出 处: 农业生物技术：英文版
• 发表日期: 2015年5期
• 关键词: 微卫星位点 杜鹃属 图书馆 SSR位点 重复序列 聚合酶链反应 快速分离 遗传多样性 Azalea （Rhododendron simsii Planch. ）
• 基金项目: Supported by Doctoral Starting up Foundation of Huanggang Normal University （2013030903） , Opening Fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Util

In this study,an( AG) n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO( fast Isolation by AFLP sequences containing repeats) method. Among 356 positive colonies verified by colony PCR of the( AG) n microsatellit