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目的 了解山东省动物宿主中小肠结肠炎耶尔森菌分布及病原学特征.方法 对从家畜和家禽宿主中分离到的菌株进行生化鉴定、血清学分型和聚合酶链反应(PCR)检测.结果 2 225份标本中共检出72株小肠结肠炎耶尔森菌,检出率为3.24%,包括生物1A型和生物4型.其中共检测到13种血清型,从猪的标本中分离到3株致病菌株,占细菌总数的4.17%,仅携带ystB基因的占81.94%.结论 本地区动物宿主中普遍存在小肠结肠炎耶尔森菌感染,本地区应进一步加强该菌的监测工作.“,”Objective To investigation the distribution and pathogenic characteristics of Yersinia enterocolitica with carring by animals in Shandong province.Method Bacteria were isolated from livestock and poultry,and detected by biochemical tests,serology,and PCR assay.Results It was found that 72 strains were isolated from 2 225 specimens,and the detection rate was 3.24 %.These strains were belonged to Biotypes 1A and Biotypes 4,from it 13 serotypes were detected.4.17% of them belonged to the pathogenic strains(isolated from swine),and 81.94 % carried ystB gene.Conclusions Yersinia enterocolitica carried by animals were prevalent in Shandong province and more attention should be paid to monitoring.