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取豚鼠离休气管约3厘米长一段,用Krebs—Henseleit溶液冲洗干净。离体气管之一端,结扎于0.1毫升刻度吸管上,另一端结扎在“乙”形金属管上,“乙”形金属管另一端与三路活塞相通,三路活塞另一端套在盛有Krebs—Henseleit溶液的注射器上,然后将气管与刻度吸管内的气泡赶掉,调节液面约于0．05刻度处,将三路活塞关上,把气管放入盛有50毫升Krebs-Henseleit溶液的麦氏玻管恒温水浴中固定,待刻度吸管内的液面稳定后再进行试验。同时在麦氏管中放入通气钩一支,以供给空气和混匀药物用。
Take the guinea pig about 3 cm long from the tracheal tube and rinse it with Krebs-Henseleit solution. One end of the isolated trachea was ligated on a 0.1 ml graduated straw and the other end was ligated on a “B” shaped metal tube. The other end of the “B” shaped metal tube was in communication with the three-way piston. The other end of the three-way piston was covered with Krebs. —Henseleit solution on the syringe, and then the gas bubbles in the trachea and graduated straw to get rid of, adjust the liquid level of about 0.05 mark, the three-way piston closed, the trachea into a 50ml Krebs-Henseleit solution of wheat The glass tube is fixed in a constant temperature water bath, and the test is performed after the liquid level in the calibration straw is stabilized. At the same time put a vent hook in the Maxwell tube to supply air and mix the drug.