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台湾农试所研究以花药培养进行全雄育种。据研究,品种及单株间花药培养之效率:①花药愈合组织自含有2mg/1NAA,1mg/1BA及6%sucrose之1/2MS基本盐类培养基( Na- Fe- EDTA全量)诱得后,先继代培养于含有1mg/1NAA及0.5 mg/1BA之Murashige et al.(1972)基本盐类培养基之处理方式,可获得最佳之分化效果;②品种及单株间花药培养效率差异极大,在80株受测芦笋雄株中,以UC500W-20单株之育种效率14.6%最高,造成这种差异的主要原因可能是各单
Taiwan Agricultural Research Institute of anther culture for all male breeding. According to the research, the varieties and the anther culture efficiency among the plants: ①The anther healing tissue was induced from 1 / 2MS basal salt medium (Na-Fe-EDTA total amount) containing 2mg / 1NAA, 1mg / 1BA and 6% sucrose , The best differentiation effect can be obtained by first subculture in the medium of Murashige et al. (1972) containing 1 mg / l NAA and 0.5 mg / lBA basal salts; (2) The difference of anther culture efficiency between cultivars and plants In the 80 tested asparagus male plants, the highest breeding efficiency of UC500W-20 was 14.6%. The main reason for this difference may be single