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用免疫细胞化学双重染色法对马桑内酯致痫大鼠齿状回及海马回ＣＡ３区内原癌基因表达、谷氨酸免疫反应的变化及其相互关系进行了研究。一侧侧脑室内注射马桑内酯诱发癫痫后，在双重免疫细胞化学染色的切片上，齿状回及海马回ＣＡ３区内均有３种不同类型的细胞：谷氨酸（Ｇｌｕ）单标细胞、Ｆｏｓ单标细胞和Ｆｏｓ／Ｇｌｕ双标细胞。癫痫发作后１ｈ，注射侧齿状回有大量Ｆｏｓ／Ｇｌｕ双标细胞，而海马回ＣＡ３区仅有散在的双标细胞；癫痫发作后１．５ｈ，海马回ＣＡ３区双标细胞数明显增多。Ｆｏｓ单标细胞数及谷氨酸免疫反应性与双标细胞数是平行的。根据以上结果，本文对马桑内酯致痫的机制进行了讨论。
Immunocytochemical double staining was used to study the changes of proto-oncogene expression and glutamate immunoreactivity in the dentate gyrus and hippocampus CA3 region of epididymitis induced by 2-Fluorouracil and its relationship with each other. One side of the intracerebroventricular injection of intranasal ephedrine induced epilepsy, double immunocytochemical staining of the slice, the dentate gyrus and hippocampal back CA3 region has three different types of cells: glutamate (Glu) single Cells, Fos single labeled cells and Fos / Glu double labeled cells. 1 h after epileptic seizures, a large number of Fos / Glu double labeled cells were injected into the dentate gyrus of the injected side, whereas double interspersed double cells were scattered in the CA3 area of the hippocampus. At 1.5 h after the seizure, the number of double labeled cells in the hippocampus CA3 increased significantly. The number of Fos single-labeled cells and glutamate immunoreactivity were in parallel with double-labeled cells. Based on the above results, this article discusses the mechanism of epilepsy induced by.