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报道了ALP,ATPase和 G-6-P在小鼠胚肝细胞和出生后 2,4,6,8,10和16周的肝细胞分化和发育过程中超微结构定位的研究。发现:小鼠胚肝发育过程中见有两种类型肝细胞:一种胞浆清沏透明,称为亮细胞;另一种胞浆呈絮状,称为暗细胞;此外,见有一种梭形细胞,胞浆含有丰富的内质网和线粒体,属间充质细胞。在上述细胞的接触面可见有电子密度很高的ALP和ATPase沿着细胞膜分布,而游离面无酶反应。出生两周以后小鼠的肝细胞结构和成年者相同;ALP和ATPase沿着毛细胆管区微绒毛分布,并且后者比前者反应更强。G-6-P沿着核膜和内质网区分布。并对这些酶出现的意义进行讨论。
We report the ultrastructural localization of ALP, ATPase and G-6-P in mouse embryonic hepatocytes and the differentiation and development of hepatocytes at 2, 4, 6, 8, 10 and 16 weeks after birth. It was found that two types of hepatocytes were found during the development of mouse embryonic liver: one kind of clear and cytoplasm clear, called bright cells; the other kind of cytoplasm was flocculent, called dark cells; in addition, there was a shuttle Cells, the cytoplasm is rich in endoplasmic reticulum and mitochondria, mesenchymal cells. In the contact surface of the above cells can be seen with high electron density of ALP and ATPase along the cell membrane distribution, while the free surface without enzyme reaction. Two weeks after birth, the mice had the same hepatocyte structure as the adults; ALP and ATPase were distributed along the microvilli of the capillary zone, and the latter reacted more strongly than the former. G-6-P is distributed along the nuclear membrane and endoplasmic reticulum. And the significance of these enzymes appear to be discussed.